• Le 15 juin 2020

Un nouveau papier de l'équipe Chimie et vectorologie paru en ligne le 15 juin 2020 dans le journal Chemistry

Tyrosine Conjugation Methods for Protein Labelling

Dimitri Alvarez DortaaDavid DeniaudaMathieu MévelbSébastien G Gouina
aEquipe Corail, CEISAM
bChimie et Vectorologie, UMR1089

Abstract

Over the last two decades, the development of chemical biology and the need for more defined protein conjugates have fostered active research on new bioconjugation techniques. In particular, a wide range of biorthogonal labelling strategies have been reported to functionalize the phenol side chain of tyrosines (Tyr). Tyr occur at medium frequency and are partially buried at the protein surface, offering interesting opportunities for site-selective labelling of the most reactive residues. Tyr-targeting has proved effective for designing a wide range of important biomolecules including antibody-drug conjugates, fluorescent or radioactive protein probes, glycovaccines, protein aggregates and PEG-conjugates. Innovative methods have also been reported for site-specific labelling with ligand-directed anchors and for specific affinity capture of proteins. This review will present and discuss these promising alternatives to the conventional labelling of the nucleophilic lysine and cysteine residues.

Keywords: 

bioconjugates * tyrosine * site-specificity * bioorthogonality * click chemistry.

Lien vers Pubmed - Liste de nos publications scientifiques